Kazushi Oda Assistant Professor
School of Materials Science（Department of Materials Science・Biofunction and Organization）
B.S.(1988), M.S.(1991) from Nagoya University, Ph.D.(1995) from Yokohama City University
NEDO Post-doctoral Research Fellow at National Institute of Bioscience and Technology(1996)
1) Cloning and manipulation of transducer genes in a purple sulfur bacterium:
Some bacteria with flagella are mobile and tactic in response to environmental stimuli. The regulatory genes of taxis are well-studied and characterized in E. coli and have remarkably consensus sequence, "Highly Conserved Domain (HCD domain)". I am now trying to clone whole genes for taxis in purple sulfur bacterium, Ectothiorhodospira halophila, by PCR with probes based on the consensus sequence. Cloned genes will be fused with green fluorescent protein (GFP) for molecular analysis.
2) Direct observation of dynamic behavior of signal transducer molecules by fluorescent microscope system:
Direct observation technique for a single molecule behavior is developed extensively. Transducer molecules fused with GFP can be observed under fluorescent microscope system and analyzed dynamic motion in vivo. Diffusion is an important process for signal transduction. Do statistical parameters play a key role in biological signal transduction? How sensitive is it for biological functions to manipulate the statistical parameters of the transducer molecules?
3) Analysis of biological motion and biological function of statistical parameters:
Using transformed bacteria, it is possible to elucidate the relationship between characteristics in one molecule of transducer and biological behavior.
◇Lectures and Presentations
- Photophobic response and methyl accepting chemotaxis protein genes in Ectothiorhodospira halophila，Kazushi Oda and Tetsuo Takahashi，Gordon Reseach Confereces "Photosensory Receptors & Signal Transduction"，April 30-May 5, 2000
- PYPを持つEctothiorhodospira halophilaの新しいトランスデューサー蛋白のクローニング，小田和司、西川彩成、高橋哲郎，第37回日本生物物理学会，1999年10月
- The Biological Society of Japan (1992-)
- The Molecular Biology Society of Japan (1991-)
- Japanese Society for Chronobiology (1994-)