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Toshifumi Tsukahara Professor
School of Transdisciplinary SciencesӢSchool of Materials ScienceӢBioscience and Biotechnology Area

¢£Degrees

B.S. Ritsumeikan University(1980),M.S. Saitama University(1984),Ph.D. from The University of Tokushima(1987)

¢£Professional Career

Post Doctoral Fellow at National Institute of Neuroscience,Japan(1987),Staff Researcher at National Institute of Neuroscience(1988), Visiting Scientist at Cold Spring Harbor Laboratory,USA(1990), Senior Staff Researcher at National Institute of Neuroscience,Japan(2002), Seccion Chif at National Institute of Neuroscience,Japan(2002)

¢£Specialties

Biochemistry, Molecular Biology, DNA Chip Technology

¢£Research Keywords

microarray, RNA splicing, gene expression, differentiation, restoration of genetic code

¢£Research Interests

(1) Development of Oligonucleotides microarray for SNPs analysis.(2) Process of differentiation of P19 cells.(3) Regulation mechanisms of alternative splicing in neuron.(4) Therapeutic approach for neurological diseases.
DNA chip enabling simultaneous analysis of a large number of genes isnow collecting public attention. In fact, the chip is quite effective forcomprehensive analyses of the genes in tissues and cells and foridentifications of known single nucleotide polymorphisms (SNPs) andmutations in genes. Therefore, the application as well as basic research ofDNA chips has rapidly progressed. At present, I am also involved in thedevelopment of new oligonuc1eotides-based DNA chips with minute porous g1assas a carrier. The aim of this study is to develop carrier-integrated probesby using porous glass as a solid phase carrier to synthesizeoligonucleotides. Once carrier-integrated probes were prepared, theaffymetrix-type DNA chip can be easily manufactured by spotting and fixingthousands carrier-integrated probes. This method seems to be epoch-making inthe sense of providing cheaper products than other methods and responding tousers”Ē requests easily. Thus, it might contribute to promote the analysesof various mutation sites in genomes such as SNPs. Using DNA chip, however, the accurate information on various proteins,that define the physiology or pathology of tissues and cells, can not beenobtained. Since in many genetic diseases, mRNAs, the first genetic products,are expressed even if small deficit and insertion or mutation is included intheir genes. This indicates that the genetic disease is difficult to bediagnosed only by the DNA chip method, and that analysis of tissue proteins,final gene products, is necessary to the diagnosis. In fact, human genomeanalysis are progressing and expected to be completed in a few years, andthen it is generally accepted that ”Čproteome”É is the most essentialproblem of post-genomic project. It is known that unlike mRNA, the proteinundergoes many post-translational modifications such as sugar chainmodification and limited proteolysis. It is also known that the functions ofmany proteins are regulated by phosphorylation. Therefore, it is necessaryto determine what kinds of proteins exist there in order to know realphysiological functions and disease states of tissues and cells. This urgesthe development of the analytical tools for proteome and devices toelaborate what kinds of proteins and where they are expressed andaccumulated in various tissues and cells.

¢£Publications

”žBooks

  • "New Developments in Alternative Splicing Research"Comprehensive Analyses of Alternative Exons in Neuronally Differentiated P19 cells_”¤Hitoshi Suzuki and Toshifumi Tsukahara”¤Nova Science Publishers, Inc”¤2013

”žPublished Papers

  • Chemical RNA Editing for Genetic Restoration: The Relationship between the Structure and Deamination Efficiency of Carboxyvinyldeoxyuridine Oligodeoxynucleotides.”¤Vu Thi Luyen, Nguyen Thi Kim Thanh, Md Thoufic Anam Azad, Hitoshi Suzuki, Toshifumi Tsukahara”¤Chemical Biology & Drug Design”¤87”¤583-593
  • Alternative splicing regulation of APP exon 7 by RBFox proteins”¤Shafiul Alam, Hitoshi Suzukia, Toshifumi Tsukahara”¤Neurochemistry International”¤in press”¤2014
  • Comprehensive analysis of alternative splicing and functionality in neuronal differentiation of P19 cells.”¤Suzuki H, Osaki K, Sano K, Alam AH, Nakamura Y, Ishigaki Y, Kawahara K, Tsukahara T.”¤PLoS One”¤6”¤2”¤e16880”¤2011

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”žLectures and Presentations

  • Possibility of Genetic Code Restoration by Chemical RNA EditingBIT's”¤Toshifumi Tsukahara, Vu Thi Luyen, Hitoshi Suzuki, and Kenzo Fujimoto”¤BIT's Biopharmaceutical Summit 2013”¤Frankfurt”¤Augst 7-8, 2013
  • Possibility of Genetic Restoration for a Disease Treatment”¤Toshifumi Tsukahara, Yu Ooka, Shafiul Alam, Hitoshi Suzuki, Kenzo Fujimoto”¤ICMSA02011”¤Kuala Lumpur, Malaysia”¤4/19”Į21, 2011

¢£Extramural Activities

”žAcademic Society Affiliations

  • The RNA Society of Japan”¤Funding Member”¤1999-
  • The RNA Society”¤Member”¤1996-
  • The Molecular Biology Society”¤Member”¤1992-

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”žOther Activities

  • BioScienceTrends, an international journal”¤Editor”¤2007/05/01 -
  • Japan Society for the Promotion of Science