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Chemical Synthesis and Cell-Free Expression of Thiazoline Ring-Bridged Cyclic Peptides and Their Properties on Biomembrane Permeability
Takashi Tamura, Masaaki Inoue, Yuji Yoshimitsu, Ichihiko Hashimoto, Noriyuki Ohashi, Kyosuke Tsumura, Koo Suzuki, Takayoshi Watanabe, Takahiro Hohsaka
Bull. Chem. Soc. Jpn., 2022, 95, 359-366.

Cell-Free Synthesis of Human Endothelin Receptors and Its Application to Ribosome Display
Hiroki Nakai, Kinuka Isshiki, Masato Hattori, Hiromasa Maehira, Tatsumi Yamaguchi, Keiko Masuda, Yoshihiro Shimizu, Takayoshi Watanabe, Takahiro Hohsaka, Wataru Shihoya, Osamu Nureki, Yasuhiko Kato, Hajime Watanabe, and Tomoaki Matsuura
Anal. Chem., 2022, 94, 3831-3839.

Bottom-up creation of an artificial cell covered with the adhesive bacterionanofiber protein AtaA
K. Noba, M. Ishikawa, A. Uyeda, T. Watanabe, T. Hohsaka, S. Yoshimoto, T. Matsuura, and K. Hori
J. Am. Chem. Soc., 2019, 141, 19058-19066.

Antigen-responsive fluorescent antibody probes generated by selective N-terminal modification of IgGs
Keisuke Fukunaga, Takayoshi Watanabe, Dian Novitasari, Hiroyuki Ohashi, Ryoji Abe, Takahiro Hohsaka
Chem. Commun.,2018, 54, 12734-12737.

Different protein localizations on the inner and outer leaflet of cell-sized liposomes using cell-free protein synthesis
A. Uyeda, T. Watanabe, T. Hohsaka, and T. Matsuura
Synth. Biol. , 2018, 3, ysy007.

Antigen-dependent fluorescence response of anti-c-Myc Quenchbody studied by molecular dynamics simulations
Yoshiharu Mori, Hisashi Okumura, Takayoshi Watanabe, Takahiro Hohsaka
Chem. Phys. Lett., 2018, 698, 223-226.

Statistical description of the denatured structure of a single protein, staphylococcal nuclease, by FRET analysis
Mariko Yamaguchi, Emi Ohta, Takuya Muto, Takayoshi Watanabe, Takahiro Hohsaka, Yoichi Yamazaki, Hironari Kamikubo and Mikio Kataoka
Biophysical Reviews,2018, 10, 145-152.

Freezing Assisted Gene Delivery Combined with Polyampholyte Nanocarriers
Sana Ahmed, Tadashi Nakaji-Hirabayashi, Takayoshi Watanabe, Takahiro Hohsaka, Kazuaki Matsumura
ACS Biomater. Sci. Eng., 2017, 3, 1677-1689.

Antibody-based fluorescent and fluorescent ratiometric indicators for detection of phosphotyrosine
K. P. Huynh Nhat, T. Watanabe, K. Yoshikoshi, T. Hohsaka
J. Biosci. Bioeng., 2016, 122, 146-154.

Double-Fluorescent-Labeled Single-Chain Antibodies Showing Antigen-Dependent Fluorescence Ratio Change
K. Yoshikoshi, T. Watanabe, T. Hohsaka
Bull. Chem. Soc. Jpn., 2016, 89, 573-580.

Incorporation of a Doubly Functionalized Synthetic Amino Acid into Proteins for Creating Chemical and Light-Induced Conjugates
A. Yamaguchi, T. Matsuda, K. Ohtake T. Yanagisawa, S. Yokoyama, Y. Fujiwara, T. Watanabe, T. Hohsaka, K. Sakamoto
Bioconjugate Chem., 2016, 27, 198-206.

Site-specific N-terminal Fluorescent Labeling of Unprotected Peptides
Keisuke Fukunaga, Takayoshi Watanabe, Takahiro Hohsaka
Peptide Science, 2016, 2015, 289-290.

Novel biosensor system model based on fluorescence quenching by a fluorescent streptavidin and carbazole-labeled biotin
Xianwei Zhu, Hiroaki Shinohara, Ryuta Miyatake, Takahiro Hohsaka
J. Mol. Recognit., 2016, 29, 485-491.

Liposome-Based in Vitro Evolution of Aminoacyl-tRNA Synthetase for Enhanced Pyrrolysine Derivative Incorporation
Atsuko Uyeda, Takayoshi Watanabe, Yasuhiko Kato, Hajime Watanabe, Tetsuya Yomo, Takahiro Hohsaka, Tomoaki Matsuura
ChemBioChem, 2015, 16, 1797-1802.

A Cell-Free Translocation System Using Extracts of Cultured Insect Cells to Yield Functional Membrane Proteins
Toru Ezure, Kei Nanatani, Yoko Sato, Satomi Suzuki, Keishi Aizawa, Satoshi Souma, Masaaki Ito, Takahiro Hohsaka, Gunnar von Heijine, Toshihiko Utsumi, Keietsu Abe, Eiji Ando, Nobuyuki Uozumi
PLoS One., 2014, 9, e112874.

Incorporation of Fluorescent Nonnatural Amino Acid into Sialic Acid-Binding Lectin for Fluorescence Detection of Ligand-Binding
Yasutaka Ito and Takahiro Hohsaka
Bull. Chem. Soc. Jpn., 2013, 86, 729-735.

Incorporation of glycosylated amino acid into protein by an in vitro translation system
Teruhiko Matsubara, Kazutoshi Iijima, Takayoshi Watanabe, Takahiro Hohsaka, Toshinori Sato
Bioorg. Med. Chem. Lett., 2013, 23, 5634-5636.

Synthesis of Novel BRET/FRET Protein Probes Containing Light-Emitting Proteins and Fluorescent Nonnatural Amino Acids.
Atsushi Yamaguchi and Takahiro Hohsaka
Bull. Chem. Soc. Jpn., 2012, 85, 576-583.

Amber Codon-Mediated Expanded Saturation Mutagenesis of Proteins using a Cell-Free Translation System.
Naoki Shozen, Takayoshi Watanabe, Takahiro Hohsaka
J. Biosci. Bioeng., 2012, 113, 704-709.

"Quenchbodies": Quench-Based Antibody Probes That Show Antigen-Dependent Fluorescence.
Ryoji Abe, Hiroyuki Ohashi, Issei Iijima, Masaki Ihara, Hiroaki Takagi, Takahiro Hohsaka, Hiroshi Ueda
J. Am. Chem. Soc., 2011, 133, 17386-17394.

Farnesyl pyrophosphate regulates adipocyte functions as an endogenous PPARgamma agonist.
Tsuyoshi Goto, Hiroyuki Nagai, Kahori Egawa, Youg-Il Kim, Sota Kato, Aki Taimatsu, Tomoya Sakamoto, Shogo Ebisu, Takahiro Hohsaka, Hiroh Miyagawa, Shigeru Murakami, Nobuyuki Takahashi, Teruo Kawada
Biochem J., 2011, 438, 111-119.

Development of a novel PPARgamma ligand screening system using pinpoint fluorescence-probed protein.
Hiroyuki Nagai, Shogo Ebisu, Ryoji Abe, Tsuyoshi Goto, Nobuyuki Takahashi, Takahiro Hohsaka, Teruo Kawada
Biosci. Biotechnol. Biochem., 2011, 75, 337-341.

Biosynthesis of proteins containing modified lysines and fluorescent labels using non-natural amino acid mutagenesis
Yasunori Tokuda, Takayoshi Watanabe, Kazushi Horiike, Kaori Shiraga, Ryoji Abe, Norihito Muranaka, Takahiro Hohsaka
J. Biosci. Bioeng., 2011, 111, 402-407.

Incorporation of fluorescent-labeled non-α-amino carboxylic acids into the N-terminus of proteins in response to amber initiation codon
Masanori Miura, Norihito Muranaka, Ryoji Abe, Takahiro Hohsaka
Bull. Chem. Soc. Jpn., 2010, 83, 546-553.

Incorporation of fluorescent non-natural amino acids into N-terminal tag of proteins in cell-free translation and its dependence on position and neighboring codons
Ryoji Abe, Kaori Shiraga, Shogo Ebisu, Hiroaki Takagi, Takahiro Hohsaka
J. Biosci. Bioeng., 2010, 110, 32-38.

Incorporation of non-natural amino acids with two labeling groups into the N-terminus of proteins
Tadayoshi Egusa, Kaori Shiraga, Takuya Chiba, Yasunori Tokuda, Issei Iijima, Takahiro Hohsaka
Bull. Chem. Soc. Jpn., 2010, 83, 176-181.

Site-specific incorporation of PEGylated amino acids into proteins using nonnatural amino acid mutagenesis
Naoki Shozen, Issei Iijima, Takahiro Hohsaka
Bioorg. Med. Chem. Lett., 2009, 19, 4909-4911.

Position-specific incorporation of fluorescent non-natural amino acids into maltose-binding protein for detection of ligand binding by FRET and fluorescence quenching
Issei Iijima, Takahiro Hohsaka
ChemBioChem, 2009, 10, 999-1006.

Comprehensive screening of amber suppressor tRNAs suitable for incorporation of non-natural amino acids in a cell-free translation system
Hikaru Taira, Yosuke Matsushita, Kenji Kojima, Kaori Shiraga, Takahiro Hohsaka
Biochem. Biophys. Res. Commun., 2008, 374, 304-308.

N-Terminal specific fluorescence labeling of proteins through incorporation of fluorescent hydroxy acid and subsequent ester cleavage
Takayoshi Watanabe, Yoichi Miyata, Ryoji Abe, Norihito Muranaka, Takahiro Hohsaka
ChemBioChem, 2008, 9, 1235-1242.

Four-base codon-mediated saturation mutagenesis in a cell-free translation system
Takayoshi Watanabe, Norihito Muranaka, Takahiro Hohsaka
J. Biosci. Bioeng., 2008, 105, 211-215.

Incorporation of unnatural non-alpha-amino acids into the N terminus of proteins in a cell-free translation system
Norihito Muranaka, Masanori Miura, Hikaru Taira, Takahiro Hohsaka
ChemBioChem, 2007, 8, 1650-1653.

Position-specific incorporation of biotinylated non-natural amino acids into a protein in a cell-free translation system
Takayoshi Watanabe, Norihito Muranaka, Issei Iijima, Takahiro Hohsaka
Biochem. Biophys. Res. Commun., 2007, 361, 794-799.

FRET analysis of protein conformational change through position-specific incorporation of fluorescent amino acids
Daisuke Kajihara, Ryoji Abe, Issei Iijima, Chie Komiyama, Masahiko Sisido, Takahiro Hohsaka
Nature Methods, 2006, 3, 923-929.

In vitro selection of tRNAs for efficient four-base decoding to incorporate non-natural amino acids into proteins in an Escherichia coli cell-free translation system
Hikaru Taira, Takahiro Hohsaka, Masahiko Sisido
Nucleic Acids Res., 2006, 34, e44.

Four-base codon mediated mRNA display to construct peptide libraries that contain multiple nonnatural amino acids
Norihito Muranaka, Takahiro Hohsaka, Masahiko Sisido
Nucleic Acids Res., 2006, 34, e7.

Binding efficiency of elongation factor Tu to tRNAs charged with nonnatural fluorescent amino acids
Hidetaka Nakata, Takashi Ohtsuki, Ryoji Abe, Takahiro Hohsaka, Masahiko Sisido
Anal. Biochem., 2006, 348, 321-323.

Synthesis and Sequence Optimization of GFP Mutants Containing Aromatic Nonnatural Amino Acids at the Tyr66 Position
Daisuke Kajihara, Takahiro Hohsaka, Masahiko Sisido
Protein Eng. Des. Sel., 2005, 18, 273-278.

Four-Base Codon-Mediated Incorporation of Nonnatural Amino Acids into Proteins in a Eukaryotic Cell-Free Transltion System
Hikaru Taira, Masaharu Fukushima, Takahiro Hohsaka, Masahiko Sisido
J. Biosci. Bioeng., 2005, 99, 473-476.

A Small-Molecule-Based Approach to Sense Codon-Templated Natural-Unnatural Hybrid Peptides. Selective Silencing and Reassignment of the Sense Codon by Orthogonal Reacylation Stalling at the Single-Codon Level
Shinsuke Sando, Keiichiro Kanatani, Nobuhiko Sato, Hironori Matsumoto, Takahiro Hohsaka, Yasuhiro Aoyama
J. Am. Chem. Soc., 2005, 127, 7998-7999.

Molecular Design of Glycoprotein Mimetics: Glycoblotting by Engineered Proteins with an Oxylamino-Functionalized Amino Acid Residue
Naoki Matsubara, Kei Oiwa, Takahiro Hohsaka, Reiko Sadamoto, Kenichi Niikura, Norio Fukuhara, Akio Takimoto, Hirosato Kondo, Shin-Ichiro Nishimura
Chem. Eur. J., 2005, 11, 6974-6981.

Position-Specific Incorporation of Dansylated Non-natural Amino Acids into Streptavidin by using a Four-Base Codon
Takahiro Hohsaka, Norihito Muranaka, Chie Komiyama, Kinue Matsui, Satomi Takaura, Ryoji Abe, Hiroshi Murakami, Masahiko Sisido
FEBS Lett., 2004, 560, 173-177.

Efficient Synthesis of Nonnatural Mutants in Escherichia coli S30 in vitro Protein Synthesizing System
Katsuhiro Yamanaka, Hidetaka Nakata, Takahiro Hohsaka, Masahiko Sisido
J. Biosci. Bioeng., 2004, 97, 395-399.

Facile Aminoacylation of pdCpA Dinucleotide with a Nonnatural Amino Acid in Cationic Micelle
Keiko Ninomiya, Tomoyoshi Kurita, Takahiro Hohsaka, Masahiko Sisido
Chem Commun (Camb)., 2003, 17, 2242-2243.

Position-Specific Incorporation of a Fluorophore-Quencher Pair into a Single Streptavidin through Orthogonal Four-Base Codon/Anticodon Pairs
Masumi Taki, Takahiro Hohsaka, Hiroshi Murakami, Kazunari Taira, Masahiko Sisido
J. Am. Chem. Soc., 2002, 124, 14586-14590.

Random Insertion and Deletion of Arbitrary Number of Bases for Codon-Based Random Mutation of DNAs
Hiroshi Murakami, Takahiro Hohsaka, Masahiko Sisido
Nature Biotechnology, 2002, 20, 76-81.

Preparation of glycosylated amino Acid derivatives for glycoprotein synthesis by in vitro translation system
Shino Manabe, Kimitoshi Sakamoto, Yoshiaki Nakahara, Masahiko Sisido, Takahiro Hohsaka, Yukishige Ito
Bioorg. Med. Chem., 2002, 10, 573-581.

Photoswitching of Peroxidase Activity by Position-Specific Incorporation of a Photoisomerizable Nonnatural Amino Acid into Horseradish Peroxidase
Norihito Muranaka, Takahiro Hohsaka, Masahiko Sisido
FEBS Lett., 2002, 510, 10-12.

A Nonnatural Amino Acid for Efficient Incorporation into Proteins as a Sensitive Fluorescent Probe
Masumi Taki, Takahiro Hohsaka, Hiroshi Murakami, Kazunari Taira, Masahiko Sisido
FEBS Lett., 2001, 507, 35-38.

Five-Base Codons for Incorporation of Nonnatural Amino Acids into Proteins
Takahiro Hohsaka, Yuki Ashizuka, Hiroshi Murakami, Masahiko Sisido
Nucleic Acids Res., 2001, 29, 3646-3651.

Incorporation of Nonnatural Amino Acids into Proteins by Using Various Four-Base Codons in an E. coli in vitro Translation System
Takahiro Hohsaka, Yuki Ashizuka, Hikaru Taira, Hiroshi Murakami, Masahiko Sisido
Biochemistry, 2001, 40,11060-11064.

Synthesis of Nonnatural Mutants of λ-Cro Repressor Protein that Contain an Electron-Accepting Amino Acid
Masahiko Sisido, Shunsuke Tokunaga, Takahiro Hohsaka
Proc. Japan Acad., 2000, 76(B), 92-96.

Sense Codon-Dependent Introduction of Unnatural Amino Acids into Multiple Sites of a Protein
Takayuki Kanda, Kazuyuki Takai, Takahiro Hohsaka, Masahiko Sisido, Hiroshi Takaku
Biochem. Biophys. Res. Commun., 2000, 270, 1136-1139.

Site-Directed Incorporation of Fluorescent Nonnatural Amino Acids into Streptavidin for Highly Sensitive Detection of Biotin
Hiroshi Murakami, Takahiro Hohsaka, Yuki Ashizuka, Kimiko Hashimoto, Masahiko Sisido
Biomacromolecules, 2000, 1, 118-125.

Incorporation of Two Different Nonnatural Amino Acids Independently into a Single Protein through Extension of the Genetic Code
Takahiro Hohsaka, Yuki Ashizuka, Hiroshi Sasaki, Hiroshi Murakami, Masahiko Sisido
J.Am.Chem.Soc., 1999, 121, 12194-12195.

Efficient Incorporation of Nonnatural Amino Acids with Large Aromatic Groups into Streptavidin in In Vitro Protein Synthesizing Systems
Takahiro Hohsaka, Daisuke Kajihara, Yuki Ashizuka, Hiroshi Murakami, Masahiko Sisido
J.Am.Chem.Soc., 1999, 121, 34-40.

Site-Directed Incorporation of p-Nitrophenylalanine into Streptavidin and Site-to-Site Photoinduced Electron Transfer from a Pyrenyl Group to a Nitrophenyl Group on the Protein Framework
Hiroshi Murakami, Takahiro Hohsaka, Yuki Ashizuka, Masahiko Sisido
J.Am.Chem.Soc., 1998, 120, 7520-7529.

Incorporation of Nonnatural Amino Acids into Streptavidin through In Vitro Frame-Shift Suppression
Takahiro Hohsaka, Yuki Ashizuka, Hiroshi Murakami, Masahiko Sisido
J.Am.Chem.Soc., 1996, 118, 9778-9779.

Site-specific Incorporation of Photofunctional Nonnatural Amino Acids into a Polypeptide through in vitro Protein Biosynthesis
Takahiro Hohsaka, Ken Sato, Masahiko Sisido, Kazuyuki Takai, Shigeyuki Yokoyama
FEBS Lett., 1994, 344, 171-174.

Photoswitching of NAD+-Mediated Enzyme Reaction through Photoreversible Antigen-Antibody Reaction
Takahiro Hohsaka, Kazuo Kawashima, Masahiko Sisido
J.Am.Chem.Soc., 1994, 116, 413-414.

Adaptability of Nonnatural Aromatic Amino Acids to the Active Center of the E. coli Ribosomal A site
Takahiro Hohsaka, Ken Sato, Masahiko Sisido, Kazuyuki Takai, Shigeyuki Yokoyama
FEBS Lett., 1993, 335, 47-50.


総説

Incorporation of Unnatural Non-α-amino Acids into the N-Terminus of Proteins
Takahiro Hohsaka
Methods Mol. Biol., 2012, 794, 229-239.

Four-base codon/anticodon strategy and non-enzymatic aminoacylation for protein engineering with non-natural amino acids
Massahiko Sisido, Keiko Ninomiya, Takashi Ohtsuki, Takahiro Hohsaka
Methods, 2005, 36, 270-278.

Incorporation of Nonnatural Amino Acids into Proteins through Extension of the Genetic Code
Takahiro Hohsaka
Bull. Chem. Soc. Jpn., 2004, 77, 1041-1049.

Random Insertion and Deletion Mutagenesis
Hiroshi Murakami, Takahiro Hohsaka, Masahiko Sisido
Methods Mol. Biol., 2003, 231, 53-64.

Incorporation of Non-natural Amino Acids into Proteins
Takahiro Hohsaka, Masahiko Sisido
Curr. Opin. Chem. Biol., 2002, 6, 809-815.

Introduction of Specialty Functions by the Position-Specific Incorporation of Nonnatural Amino Acids into Proteins through Four-Base Codon/Anticodon Pairs
Masahiko Sisido and Takahiro Hohsaka
Appl. Microbiol. Biotechnol., 2001, 57, 274ミ281.

Extension of Protein Functions by the Incorporation of Nonnatural Amino Acids
Masahiko Sisido, Takahiro Hohsaka
Bull. Chem. Soc. Jpn., 1999, 72, 1409-1425.


和文総説

遺伝暗号とタンパク質機能の拡張
芳坂貴弘
高分子, 2014, 63(10), 722-723.

非天然アミノ酸のタンパク質への導入技術−バイオメディカル応用に向けて
芳坂貴弘
メディカルバイオ別冊, 2010, 72-77.

無細胞翻訳系における非天然アミノ酸の導入技術
芳坂貴弘
生化学, 2007, 79(3), 247-253.

遺伝暗号を拡張した人工タンパク質合成系の開発と応用
芳坂貴弘
生物物理, 2007, 47(2), 124-128.

遺伝暗号を拡張した人工タンパク質合成システム
芳坂貴弘
メディカル・サイエンス・ダイジェスト, 2007, 33(3), 744-745.

4塩基コドンを用いたタンパク質のピンポイント蛍光標識法
芳坂貴弘
実験医学, 2007, 25(1), 81-85.

遺伝暗号を拡張した人工タンパク質合成システム
芳坂貴弘
現代化学, 2007, 430, 58-62.(1月号)

人工タンパク質合成システムの開発と応用
芳坂貴弘
化学工業, 2006, 57(9), 707-711.

非天然アミノ酸の導入による蛋白質の蛍光ラベル法とその応用
宍戸昌彦、瀧 真清、大槻高史、芳坂貴弘
蛋白質核酸酵素, 2006, 51(5), 399-407.

遺伝暗号を拡張して人工蛋白質を合成する
芳坂貴弘
化学と生物, 2005, 43(11), 753-757.

次世代のタンパク質改変技術−非天然アミノ酸の導入技術の開発と応用−
芳坂貴弘
化学工業, 2005, 56(11), 884-888.

DDS研究のためのタンパク質工学技術
〜遺伝暗号の拡張による非天然型アミノ酸のタンパク質への導入〜
芳坂貴弘
Bioベンチャー, 2004, 4(6), 69-71.(11-12月号)

遺伝暗号を拡張して新しいタンパク質をつくる
芳坂貴弘
現代化学, 2004, 397, 24-29.(4月号)

新規変異タンパク質創出のためのランダム挿入削除変異法
村上裕、芳坂貴弘、宍戸昌彦
Bioベンチャー, 2002, 2(4), 94-97.(7-8月号)

化学のフロンティア2000
遺伝暗号を拡張した人工タンパク質合成システム
−化学と遺伝子工学の融合を目指して−
芳坂貴弘
化学と工業, 2000, 53, 169-172.

非天然アミノ酸を部位特異的に導入した電子機能性タンパク質の創製
篠原寛明、芳坂貴弘、宍戸昌彦
Electrochemistry, 2000, 68, 352-355.

機能性非天然アミノ酸を含む蛋白質の合成と応用
宍戸昌彦、芳坂貴弘
化学と工業, 1996, 49, 515-517.

著書

変異導入法
芳坂貴弘
揺らぎ・ダイナミクスと生体機能(寺嶋正秀編), p91-102, 化学同人, 2013.

人工タンパク質の合成と応用
芳坂貴弘
超分子サイエンス&テクノロジー(国武豊喜監修), p890-895, エヌティーエス, 2009.

非天然型アミノ酸を含むタンパク質
芳坂貴弘
トコトンやさしいタンパク質の本(東京工業大学生命理工学研究科編), p112-113, 日刊工業新聞社, 2007.

非天然アミノ酸導入
芳坂貴弘
生物工学ハンドブック(日本生物工学会編), p178-188, コロナ社, 2005.

人工タンパク質
芳坂貴弘
高分子新素材のすべて(国武豊喜監修), p138-141, 工業調査会, 2005.

非天然アミノ酸の導入
芳坂貴弘
コンビナトリアルバイオエンジニアリングの最前線(植田充美監修), p178-188, シーエムシー出版, 2004.

人工コドン
芳坂貴弘、宍戸昌彦
生命化学のニューセントラルドグマ(杉本直己編), p96-103, 化学同人, 2002.