Development of automated competitive ELISA paper-based analytical device using dissolvable sucrose valves for Aflatoxin B1 detection
Charernchai Sumamal, Miyuki Chikae，Wanida Wonsawat，Tue Phan Trong，Yuzuru Takamura
A fully automated competitive enzyme linked immunosorbent assay (ELISA) was established on a laser-cut paper-based device (µPAD) integrated with sucrose valve for the detection of a small-sized target, aflatoxin B1 (AFB1), which is a carcinogenic agent. Previously, our group reported automated µPAD for sandwich ELISA using inkjet printing (Apilux et al., 2013), however, which was not applicable for small-sized target and required complicated and time-consuming fabrication. Thus, faster and simpler techniques capable of high-precision mass production is a crucial need in order for µPADs to provide widespread affordable access to analytical tools. In this study, the conventional laser platform is introduced to remove the hydrophilic nitrocellulose membrane (NCM) creating a hydrophobic barrier along the edge of the cutline. Using laser cutting, an ease of use, accuracy, reproducibility and device production speed were improved (30 times faster than inkjet printing). To automate the multistep processes of competitive ELISA, sequential delivery of reagents was achieved with sucrose valve, which was designed to open spontaneously after a given delay, and was occupying smaller area with simpler structure compared to the winding path method for making delay used in previous work.
It is a great honor for me to receive the Young Scientist Presentation Award at the 80th JSAP Autumn Meeting, 2019. This is one of the most significant events of my research, academic and life's experience. It would not be possible for me to achieve this award without the constant support of many important people. Foremost, my deepest gratitude goes to Prof. Yuzuru Takamura for his great effort, generosity and guidance. Special thanks goes to Dr. Miyuki Chikae and Asst. Prof. Phan Trong Tue for their attention and advice. Last but not least, many thanks go to my family for their undying love and encouragement in me. This honorable and memorable moment will be motivation for all my future activities. Thank you so much.